Dielectric permittivity capable of vitrification cryoprotective media

Abstract

The research aim was to determine dielectric parameters and state of water in capable of vitrification media PVS2, 88% PVS3 and PVSN, the components of those are glycerol, dimethyl sulfoxide, ethylene glycol and non-penetrating cryoprotectant sucrose. These media are widely used to cryopreserve meristems, embryoids, callus, allowing the preservation of plant genetic resources under liquid nitrogen conditions for a long time period. Dielectric characteristics of the samples of cryoprotective media, their real ε′ and imaginary ε′′ components were measured at 20 °C using resonant type UHF-dielectrometer at 9.2 GHz frequency. The values of static dielectric permittivity εs of the media on the low-frequency side of the water relaxation and dielectric relaxation frequency of water molecules fd were counted using the Debye equations. It has been shown that the least values of dielectric parameters are characteristic for 88% cryoprotective medium PVS3, which comprises such cryoprotectants as glycerol and sucrose. This medium has the highest ability of binding and ordering the water. When determining the cytotoxicity and effect of low temperature storage on viability of potato, grape, garlic meristems all the studied cryoprotective media can be used when developing the efficient protocols of cryopreservation.

Keywords

• кріозахисні середовища
• меристема
• гліцерин
• диметилсульфоксид
• етиленгліколь
• сахароза
• НВЧ-діелектрометрія