Nature Communications (Jul 2021)
BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons
- Johannes Vierock,
- Silvia Rodriguez-Rozada,
- Alexander Dieter,
- Florian Pieper,
- Ruth Sims,
- Federico Tenedini,
- Amelie C. F. Bergs,
- Imane Bendifallah,
- Fangmin Zhou,
- Nadja Zeitzschel,
- Joachim Ahlbeck,
- Sandra Augustin,
- Kathrin Sauter,
- Eirini Papagiakoumou,
- Alexander Gottschalk,
- Peter Soba,
- Valentina Emiliani,
- Andreas K. Engel,
- Peter Hegemann,
- J. Simon Wiegert
Affiliations
- Johannes Vierock
- Institute for Biology, Experimental Biophysics, Humboldt University Berlin
- Silvia Rodriguez-Rozada
- Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf
- Alexander Dieter
- Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf
- Florian Pieper
- Department of Neurophysiology and Pathophysiology, University Medical Center Hamburg-Eppendorf
- Ruth Sims
- Wavefront-Engineering Microscopy Group, Photonics Department, Institut de la Vision, Sorbonne Université, INSERM, CNRS, Institut de la Vision
- Federico Tenedini
- Research Group Neuronal Patterning and Connectivity, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf
- Amelie C. F. Bergs
- Buchmann Institute for Molecular Life Sciences and Institute of Biophysical Chemistry, Goethe University
- Imane Bendifallah
- Wavefront-Engineering Microscopy Group, Photonics Department, Institut de la Vision, Sorbonne Université, INSERM, CNRS, Institut de la Vision
- Fangmin Zhou
- Research Group Neuronal Patterning and Connectivity, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf
- Nadja Zeitzschel
- Buchmann Institute for Molecular Life Sciences and Institute of Biophysical Chemistry, Goethe University
- Joachim Ahlbeck
- Department of Neurophysiology and Pathophysiology, University Medical Center Hamburg-Eppendorf
- Sandra Augustin
- Institute for Biology, Experimental Biophysics, Humboldt University Berlin
- Kathrin Sauter
- Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf
- Eirini Papagiakoumou
- Wavefront-Engineering Microscopy Group, Photonics Department, Institut de la Vision, Sorbonne Université, INSERM, CNRS, Institut de la Vision
- Alexander Gottschalk
- Buchmann Institute for Molecular Life Sciences and Institute of Biophysical Chemistry, Goethe University
- Peter Soba
- Research Group Neuronal Patterning and Connectivity, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf
- Valentina Emiliani
- Wavefront-Engineering Microscopy Group, Photonics Department, Institut de la Vision, Sorbonne Université, INSERM, CNRS, Institut de la Vision
- Andreas K. Engel
- Department of Neurophysiology and Pathophysiology, University Medical Center Hamburg-Eppendorf
- Peter Hegemann
- Institute for Biology, Experimental Biophysics, Humboldt University Berlin
- J. Simon Wiegert
- Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf
- DOI
- https://doi.org/10.1038/s41467-021-24759-5
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 20
Abstract
Currently, bidirectional control of activity in the same neurons in the same experiment is difficult. Here the authors report a Bidirectional Pair of Opsins for Light-induced Excitation and Silencing, BiPOLES, which they use in a range of organisms including worms, fruit flies, mice and ferrets.
