Serum amyloid A and mitochondrial DNA in extracellular vesicles are novel markers for detecting traumatic brain injury in a mouse model

Tony Z. Tang; Yingxin Zhao; Deepesh Agarwal; Aabila Tharzeen; Igor Patrikeev; Yuanyi Zhang; Jana DeJesus; Stefan H. Bossmann; Balasubramaniam Natarajan; Massoud Motamedi; Bartosz Szczesny

iScience (Feb 2024)

Serum amyloid A and mitochondrial DNA in extracellular vesicles are novel markers for detecting traumatic brain injury in a mouse model

Tony Z. Tang,
Yingxin Zhao,
Deepesh Agarwal,
Aabila Tharzeen,
Igor Patrikeev,
Yuanyi Zhang,
Jana DeJesus,
Stefan H. Bossmann,
Balasubramaniam Natarajan,
Massoud Motamedi,
Bartosz Szczesny

Affiliations

Tony Z. Tang: Department of Ophthalmology and Visual Sciences, University of Texas Medical Branch, Galveston, TX, USA
Yingxin Zhao: Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX, USA
Deepesh Agarwal: Department of Electrical and Computer Engineering, Kansas State University, Manhattan, KS, USA
Aabila Tharzeen: Department of Electrical and Computer Engineering, Kansas State University, Manhattan, KS, USA
Igor Patrikeev: Department of Ophthalmology and Visual Sciences, University of Texas Medical Branch, Galveston, TX, USA
Yuanyi Zhang: Department of Office of Biostatistics, University of Texas Medical Branch, Galveston, TX, USA
Jana DeJesus: Department of Surgery, University of Texas Medical Branch, Galveston, TX, USA
Stefan H. Bossmann: Department of Cancer Biology, University of Kansas Medical Center, Kansas City, KS, USA
Balasubramaniam Natarajan: Department of Electrical and Computer Engineering, Kansas State University, Manhattan, KS, USA
Massoud Motamedi: Department of Ophthalmology and Visual Sciences, University of Texas Medical Branch, Galveston, TX, USA
Bartosz Szczesny: Department of Ophthalmology and Visual Sciences, University of Texas Medical Branch, Galveston, TX, USA; Department of Anesthesiology, University of Texas Medical Branch, Galveston, TX, USA; Corresponding author

Journal volume & issue: Vol. 27, no. 2
p. 108932

Abstract

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Summary: This study investigates the potential use of circulating extracellular vesicles’ (EVs) DNA and protein content as biomarkers for traumatic brain injury (TBI) in a mouse model. Despite an overall decrease in EVs count during the acute phase, there was an increased presence of exosomes (CD63+ EVs) during acute and an increase in microvesicles derived from microglia/macrophages (CD11b+ EVs) and astrocytes (ACSA-2+ EVs) in post-acute TBI phases, respectively. Notably, mtDNA exhibited an immediate elevation post-injury. Neuronal (NFL) and microglial (Iba1) markers increased in the acute, while the astrocyte marker (GFAP) increased in post-acute TBI phases. Novel protein biomarkers (SAA, Hp, VWF, CFD, CBG) specific to different TBI phases were also identified. Biostatistical modeling and machine learning identified mtDNA and SAA as decisive markers for TBI detection. These findings emphasize the importance of profiling EVs’ content and their dynamic release as an innovative diagnostic approach for TBI in liquid biopsies.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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