Opposite Effects of mRNA-Based and Adenovirus-Vectored SARS-CoV-2 Vaccines on Regulatory T Cells: A Pilot Study

Francesca La Gualana; Francesca Maiorca; Ramona Marrapodi; Francesca Villani; Marzia Miglionico; Stefano Angelo Santini; Fabio Pulcinelli; Laura Gragnani; Silvia Piconese; Massimo Fiorilli; Stefania Basili; Milvia Casato; Lucia Stefanini; Marcella Visentini

doi:10.3390/biomedicines11020511

Biomedicines (Feb 2023)

Opposite Effects of mRNA-Based and Adenovirus-Vectored SARS-CoV-2 Vaccines on Regulatory T Cells: A Pilot Study

Francesca La Gualana,
Francesca Maiorca,
Ramona Marrapodi,
Francesca Villani,
Marzia Miglionico,
Stefano Angelo Santini,
Fabio Pulcinelli,
Laura Gragnani,
Silvia Piconese,
Massimo Fiorilli,
Stefania Basili,
Milvia Casato,
Lucia Stefanini,
Marcella Visentini

Affiliations

Francesca La Gualana: Department of Translational and Precision Medicine, Sapienza University of Rome, Viale dell’Università 37, 00185 Rome, Italy
Francesca Maiorca: Department of Translational and Precision Medicine, Sapienza University of Rome, Viale dell’Università 37, 00185 Rome, Italy
Ramona Marrapodi: Department of Translational and Precision Medicine, Sapienza University of Rome, Viale dell’Università 37, 00185 Rome, Italy
Francesca Villani: Department of Translational and Precision Medicine, Sapienza University of Rome, Viale dell’Università 37, 00185 Rome, Italy
Marzia Miglionico: Department of Translational and Precision Medicine, Sapienza University of Rome, Viale dell’Università 37, 00185 Rome, Italy
Stefano Angelo Santini: Department of Basic, Clinical, Intensive and Perioperative Biotechnological Sciences, Catholic University, Largo Agostino Gemelli 8, 00168 Rome, Italy
Fabio Pulcinelli: Department of Experimental Medicine, Sapienza University of Rome, Viale dell’Università 37, 00185 Rome, Italy
Laura Gragnani: MASVE Interdepartmental Center, Department of Experimental and Clinical Medicine, University of Florence, Largo Brambilla 3, 50134 Florence, Italy
Silvia Piconese: Department of Internal Clinical Sciences, Anaesthesiology and Cardiovascular Sciences, Sapienza University of Rome, Viale dell’Università 37, 00185 Rome, Italy
Massimo Fiorilli: Department of Translational and Precision Medicine, Sapienza University of Rome, Viale dell’Università 37, 00185 Rome, Italy
Stefania Basili: Department of Translational and Precision Medicine, Sapienza University of Rome, Viale dell’Università 37, 00185 Rome, Italy
Milvia Casato: Department of Translational and Precision Medicine, Sapienza University of Rome, Viale dell’Università 37, 00185 Rome, Italy
Lucia Stefanini: Department of Translational and Precision Medicine, Sapienza University of Rome, Viale dell’Università 37, 00185 Rome, Italy
Marcella Visentini: Department of Translational and Precision Medicine, Sapienza University of Rome, Viale dell’Università 37, 00185 Rome, Italy

DOI: https://doi.org/10.3390/biomedicines11020511
Journal volume & issue: Vol. 11, no. 2
p. 511

Abstract

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New-generation mRNA and adenovirus vectored vaccines against SARS-CoV-2 spike protein are endowed with immunogenic, inflammatory and immunomodulatory properties. Recently, BioNTech developed a noninflammatory tolerogenic mRNA vaccine (MOGm1Ψ) that induces in mice robust expansion of antigen-specific regulatory T (Treg) cells. The Pfizer/BioNTech BNT162b2 mRNA vaccine against SARS-CoV-2 is identical to MOGm1Ψ except for the lipid carrier, which differs for containing lipid nanoparticles rather than lipoplex. Here we report that vaccination with BNT162b2 led to an increase in the frequency and absolute count of CD4posCD25highCD127low putative Treg cells; in sharp contrast, vaccination with the adenovirus-vectored ChAdOx1 nCoV-19 vaccine led to a significant decrease of CD4posCD25high cells. This pilot study is very preliminary, suffers from important limitations and, frustratingly, very hardly can be refined in Italy because of the >90% vaccination coverage. Thus, the provocative perspective that BNT162b2 and MOGm1Ψ may share the capacity to promote expansion of Treg cells deserves confirmatory studies in other settings.

Published in Biomedicines

ISSN: 2227-9059 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: http://www.mdpi.com/journal/biomedicines

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