High Resolution Melting Curve Method for Detection of BIM Deletion

Jinjing XIA; Hao BAI; Liwen XIONG; Rong LI; Bo YAN; Minhua SHAO; Baohui HAN

doi:10.3779/j.issn.1009-3419.2014.03.10

Chinese Journal of Lung Cancer (Mar 2014)

High Resolution Melting Curve Method for Detection of BIM Deletion

Jinjing XIA,
Hao BAI,
Liwen XIONG,
Rong LI,
Bo YAN,
Minhua SHAO,
Baohui HAN

Affiliations

Jinjing XIA: Department of Respiratory Medicine, Shanghai Chest Hospital, School of Medicine, Shanghai Jiaotong University,  Shanghai 200030, China
Hao BAI: Department of Respiratory Medicine, Shanghai Chest Hospital, School of Medicine, Shanghai Jiaotong University,  Shanghai 200030, China
Liwen XIONG: Department of Respiratory Medicine, Shanghai Chest Hospital, School of Medicine, Shanghai Jiaotong University,  Shanghai 200030, China
Rong LI: Department of Respiratory Medicine, Shanghai Chest Hospital, School of Medicine, Shanghai Jiaotong University,  Shanghai 200030, China
Bo YAN: Department of Respiratory Medicine, Shanghai Chest Hospital, School of Medicine, Shanghai Jiaotong University,  Shanghai 200030, China
Minhua SHAO: Department of Respiratory Medicine, Shanghai Chest Hospital, School of Medicine, Shanghai Jiaotong University,  Shanghai 200030, China
Baohui HAN: Department of Respiratory Medicine, Shanghai Chest Hospital, School of Medicine, Shanghai Jiaotong University,  Shanghai 200030, China

DOI: https://doi.org/10.3779/j.issn.1009-3419.2014.03.10
Journal volume & issue: Vol. 17, no. 3
pp. 238 – 242

Abstract

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Background and objective The aim of this study is to establish a HRM (high resolution melting curve) method for detection of deletion in human BIM gene and to detect this site deletion with the above method in 30 lung cancer samples and 30 normal samples. Methods The primers for detection of BIM deletion were designed and synthesized. The HRM method for geng deletion was established. And select the part of samples to detect BIM delection by normal PCR and sequencing assay. The Tm value of wild type PCR products was higher than that of the deletion PCR products. The difference of the corresponding Tm value is 2.5 oC. Results By detection with HRM methods, 1 samples were confirmed to be mutant, 7 samples were confirmed to be heterozygous and the other 22 samples were all wild type in the lung cancer samples. 2 samples were confirmed to be heterozygous and the other 28 samples were all wild type in the normal samples. Conclusion The HRM method for detection of BIM deletion established in this study is a sensitive, accurate, simple and high throughput method.

Published in Chinese Journal of Lung Cancer

ISSN: 1009-3419 (Print); 1999-6187 (Online)
Publisher: Chinese Anti-Cancer Association; Chinese Antituberculosis Association
Country of publisher: China
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: http://www.lungca.org

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