APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES

Abstract

Abstract. Present work describes a multiplex “sandvich” approach to enzyme-linked, solid-phase immunosorbent assay designed in microarray formate, aiming for quantitative evaluation of individual profile of cytokine expression. Its high specificity, throughput and sensitivity (30 to 20,000 pg/mL) are demonstrated with human IL-2, IL-4, IL-10, and TNF assays. This diagnostic technique may be further used both for research applications and in clinical trials.

Keywords

• cytokines
• antibodies
• microarray
• immunological analysis