Enzymatic synthesis of benzylisoquinoline alkaloids using a parallel cascade strategy and tyrosinase variants

Yu Wang; Fabiana Subrizi; Eve M. Carter; Tom D. Sheppard; John M. Ward; Helen C. Hailes

doi:10.1038/s41467-022-33122-1

Nature Communications (Sep 2022)

Enzymatic synthesis of benzylisoquinoline alkaloids using a parallel cascade strategy and tyrosinase variants

Yu Wang,
Fabiana Subrizi,
Eve M. Carter,
Tom D. Sheppard,
John M. Ward,
Helen C. Hailes

Affiliations

Yu Wang: Department of Chemistry, Christopher Ingold Building, University College London
Fabiana Subrizi: Department of Chemistry, Christopher Ingold Building, University College London
Eve M. Carter: Department of Chemistry, Christopher Ingold Building, University College London
Tom D. Sheppard: Department of Chemistry, Christopher Ingold Building, University College London
John M. Ward: Department of Biochemical Engineering, Bernard Katz Building, University College London
Helen C. Hailes: Department of Chemistry, Christopher Ingold Building, University College London

DOI: https://doi.org/10.1038/s41467-022-33122-1
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

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Pharmaceuticals derived from benzylisoquinoline alkaloids find application in modern medicine. Here, the authors report the design of a parallel enzymatic cascade to generate halogenated benzylisoquinoline alkaloids, that included engineering of tyrosinase mutants to enhance substrate capabilities.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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