Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF

Abstract

Matrix-assisted laser desorption/ionisation spiral orbit-type time-of-flight mass spectrometry (MALDI-SpiralTOF) can analyse lipid profiles and characterise lipid structure. Imaging mass spectrometry (IMS) also provides distribution maps of selected m/z values. Here, we investigated triacylglycerol (TG) structure and distribution using these technologies to estimate mouse fatty liver. The distribution and intensity of the most intense mass spectrum ion was indicated by IMS at m/z 881.7 (52:2). Analysis using MS/MS showed a structural change between liver TG and dietary TG. These findings suggest that MALDI-SpiralTOF is a powerful tool for clinical screening and estimating fatty liver.

Keywords

• Fatty liver
• Triacylglycerol
• Matrix-assisted laser desorption/ionisation
• Spiral orbit-type time-of-flight mass spectrometer
• Imaging mass spectrometry