NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens

Yulia A. Bogdanova; Elvira R. Zaitseva; Alexander Yu. Smirnov; Nadezhda S. Baleeva; Alexey S. Gavrikov; Ivan N. Myasnyanko; Sergey A. Goncharuk; Erik F. Kot; Konstantin S. Mineev; Alexander S. Mishin; Mikhail S. Baranov

doi:10.3390/ijms24097958

International Journal of Molecular Sciences (Apr 2023)

NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens

Yulia A. Bogdanova,
Elvira R. Zaitseva,
Alexander Yu. Smirnov,
Nadezhda S. Baleeva,
Alexey S. Gavrikov,
Ivan N. Myasnyanko,
Sergey A. Goncharuk,
Erik F. Kot,
Konstantin S. Mineev,
Alexander S. Mishin,
Mikhail S. Baranov

Affiliations

Yulia A. Bogdanova: Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
Elvira R. Zaitseva: Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
Alexander Yu. Smirnov: Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
Nadezhda S. Baleeva: Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
Alexey S. Gavrikov: Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
Ivan N. Myasnyanko: Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
Sergey A. Goncharuk: Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
Erik F. Kot: Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
Konstantin S. Mineev: Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
Alexander S. Mishin: Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
Mikhail S. Baranov: Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia

DOI: https://doi.org/10.3390/ijms24097958
Journal volume & issue: Vol. 24, no. 9
p. 7958

Abstract

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In this work, we showed that the well-known NanoLuc luciferase can act as a fluorogen activating protein for various arylidene-imidazolones structurally similar to the Kaede protein chromophore. We showed that such compounds can be used as fluorescent sensors for this protein and can also be used in pairs with it in fluorescent microscopy as a genetically encoded tag.

Published in International Journal of Molecular Sciences

ISSN: 1661-6596 (Print); 1422-0067 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General); Science: Chemistry
Website: http://www.mdpi.com/journal/ijms

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