Tzu-Chi Medical Journal (May 2024)

Enhancing recombinant antibody yield in Chinese hamster ovary cells

  • Chee-Hing Yang,
  • Hui-Chun Li,
  • Shih-Yen Lo

DOI
https://doi.org/10.4103/tcmj.tcmj_315_23
Journal volume & issue
Vol. 36, no. 3
pp. 240 – 250

Abstract

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A range of recombinant monoclonal antibodies (rMAbs) have found application in treating diverse diseases, spanning various cancers and immune system disorders. Chinese hamster ovary (CHO) cells have emerged as the predominant choice for producing these rMAbs due to their robustness, ease of transfection, and capacity for posttranslational modifications akin to those in human cells. Transient transfection and/or stable expression could be conducted to express rMAbs in CHO cells. To bolster the yield of rMAbs in CHO cells, a multitude of approaches have been developed, encompassing vector optimization, medium formulation, cultivation parameters, and cell engineering. This review succinctly outlines these methodologies when also addressing challenges encountered in the production process, such as issues with aggregation and fucosylation.

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