Associations of <i>TRAF2</i> (rs867186), <i>TAB2</i> (rs237025), <i>IKBKB</i> (rs13278372) Polymorphisms and <i>TRAF2</i>, <i>TAB2</i>, <i>IKBKB</i> Protein Levels with Clinical and Morphological Features of Pituitary Adenomas

Abstract

Aim: The aim of this study was to determine associations of TRAF2 (rs867186), TAB2 (rs237025), IKBKB (rs13278372) gene polymorphisms and TRAF2, TAB2, IKBKB protein levels with clinical and morphological features of pituitary adenomas (PAs). Methods: This case–control study included 459 individuals divided into two groups: a control group (n = 320) and a group of individuals with PAs (n = 139). DNA from peripheral blood leukocytes was isolated using salt precipitation and column method. Real-time PCR was used for TRAF2 (rs867186), TAB2 (rs237025), and IKBKB (rs13278372) SNP genotyping, and TRAF2, TAB2, IKBKB protein concentration measurements were performed by immunoenzymatic analysis tests using a commercial ELISA kit according to the manufacturer’s recommendations. The labeling index Ki-67 was determined by immunohistochemical analysis using a monoclonal antibody (clone SP6; Spring Bioscience Corporation). Statistical data analysis was performed using the programs "IMB SPSS Statistics 29.0". Results: We found significant differences in TRAF2 (rs867186) genotypes (AA, AG, GG) between groups: 79.1%, 17.3%, 3.6% vs. 55.3%, 20.9%, 23.8% (p p p p p p p p p p = 0.001), and each G allele reduced microadenoma odds by 3.1-fold (p p p p p TRAF2 (rs867186) G allele and GG genotype are significantly associated with reduced odds of pituitary adenomas, including both microadenomas and macroadenomas, compared to the AA genotype. These findings suggest a protective role of the G allele against the occurrence of these tumors.

Keywords

• pituitary adenoma
• gene polymorphisms <i>TRAF2</i> (rs867186)
• <i>TAB2</i> (rs237025)
• <i>IKBKB</i> (rs13278372) ELISA
• <i>TRAF2</i>
• <i>TAB2</i>