3D Printed Microfluidic Cell for SAXS Time-Resolved Measurements of the Structure of Protein Crystallization Solutions

Margarita A. Marchenkova; Sergei V. Chapek; Petr V. Konarev; Ksenia B. Ilina; Georgy S. Peters; Yury V. Pisarevsky; Vladimir A. Shishkov; Alexander V. Soldatov; Mikhail V. Kovalchuk

doi:10.3390/cryst13060938

Crystals (Jun 2023)

3D Printed Microfluidic Cell for SAXS Time-Resolved Measurements of the Structure of Protein Crystallization Solutions

Margarita A. Marchenkova,
Sergei V. Chapek,
Petr V. Konarev,
Ksenia B. Ilina,
Georgy S. Peters,
Yury V. Pisarevsky,
Vladimir A. Shishkov,
Alexander V. Soldatov,
Mikhail V. Kovalchuk

Affiliations

Margarita A. Marchenkova: A.V. Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, 59, Leninskii Prospect, 119333 Moscow, Russia
Sergei V. Chapek: The Smart Materials Research Institute, Southern Federal University, 178/124, Andreya Sladkova Street, 344090 Rostov on Don, Russia
Petr V. Konarev: A.V. Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, 59, Leninskii Prospect, 119333 Moscow, Russia
Ksenia B. Ilina: A.V. Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, 59, Leninskii Prospect, 119333 Moscow, Russia
Georgy S. Peters: National Research Centre “Kurchatov Institute”, 1, Akademika Kurchatova pl., 123182 Moscow, Russia
Yury V. Pisarevsky: A.V. Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, 59, Leninskii Prospect, 119333 Moscow, Russia
Vladimir A. Shishkov: A.V. Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, 59, Leninskii Prospect, 119333 Moscow, Russia
Alexander V. Soldatov: The Smart Materials Research Institute, Southern Federal University, 178/124, Andreya Sladkova Street, 344090 Rostov on Don, Russia
Mikhail V. Kovalchuk: A.V. Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, 59, Leninskii Prospect, 119333 Moscow, Russia

DOI: https://doi.org/10.3390/cryst13060938
Journal volume & issue: Vol. 13, no. 6
p. 938

Abstract

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A multichannel microfluidic cell (MFC) obtained using 3D printing for studying the structure of complex solutions by small-angle X-ray scattering (SAXS) is described. MFC was tested at the BioMUR beamline of the Kurchatov synchrotron. A comparative analysis of SAXS signal from the standard capillary and from the developed MFC was carried out, with MFC showing significant advantages. The dynamics of SAXS scattering curves for lysozyme solutions with NaCl precipitant were studied when the protein and precipitant concentrations changed. The obtained time series of data are well consistent with the known data for the lysozyme solution.

Published in Crystals

ISSN: 2073-4352 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Crystallography
Website: http://www.mdpi.com/journal/crystals/

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