Antimicrobial Activity and Phytochemical Analysis of Endophytic Fungal Extracts Isolated from Ethno-Pharmaceutical Plant Rauwolfia tetraphylla L.

Abstract

Endophytes are the mimicking the plant secondary metabolites as well as vast number chemical synthesizers inside plants and also have been extensively investigated for their endophytic microbial complement. Different parts of Rauwolfia tetraphylla L were subjected to the isolation of endophytic fungi. The isolated endophytic fungi were identified morphologically and, isolation, colonization rates and relative frequency of various isolates were calculated. The endophytic fungal isolates were screened for antimicrobial activity against Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium and Candida albicans. The identification of the prospective endophytic fungi Curvularia sp. (RTFs-6) and Aspergillus sp. (RTL-6) were selected for the production of secondary metabolites which were grown in rice medium and extracted with ethyl acetate and were screened for their antimicrobial activity by agar well diffusion method and Phytochemical analysis. The extract of Curvularia sp. gave effective inhibition to the all the tested organisms whereas extract of Aspergillus sp. gave effective inhibition against tested organisms except P. aeruginosa. Phytochemical compounds screening of the extracts revealed the presence of alkaloids, terpenoids and tannin compounds.

Keywords

• rauwolfia tetraphylla l
• endophytic fungi
• colonization rate
• isolation rate
• antimicrobial
• phytochemicals.