Exercise Training Decreases Nitrite Concentration in the Heart and Locomotory Muscles of Rats Without Changing the Muscle Nitrate Content

Abstract

Background Skeletal muscles are postulated to be a potent regulator of systemic nitric oxide homeostasis. In this study, we aimed to evaluate the impact of physical training on the heart and skeletal muscle nitric oxide bioavailability (judged on the basis of intramuscular nitrite and nitrate) in rats. Methods and Results Rats were trained on a treadmill for 8 weeks, performing mainly endurance running sessions with some sprinting runs. Muscle nitrite (NO2−) and nitrate (NO3−) concentrations were measured using a high‐performance liquid chromatography–based method, while amino acids, pyruvate, lactate, and reduced and oxidized glutathione were determined using a liquid chromatography coupled with tandem mass spectrometry technique. The content of muscle nitrite reductases (electron transport chain proteins, myoglobin, and xanthine oxidase) was assessed by western immunoblotting. We found that 8 weeks of endurance training decreased basal NO2− in the locomotory muscles and in the heart, without changes in the basal NO3−. In the slow‐twitch oxidative soleus muscle, the decrease in NO2− was already present after the first week of training, and the content of nitrite reductases remained unchanged throughout the entire period of training, except for the electron transport chain protein content, which increased no sooner than after 8 weeks of training. Conclusions Muscle NO2− level, opposed to NO3−, decreases in the time course of training. This effect is rapid and already visible in the slow‐oxidative soleus after the first week of training. The underlying mechanisms of training‐induced muscle NO2− decrease may involve an increase in the oxidative stress, as well as metabolite changes related to an increased muscle anaerobic glycolytic activity contributing to (1) direct chemical reduction of NO2− or (2) activation of muscle nitrite reductases.

Keywords

• endurance training
• exercise tolerance
• nitrite reductases
• skeletal muscles