Molecular Systems Biology (Sep 2011)
Arabidopsis G‐protein interactome reveals connections to cell wall carbohydrates and morphogenesis
- Karsten Klopffleisch,
- Nguyen Phan,
- Kelsey Augustin,
- Robert S Bayne,
- Katherine S Booker,
- Jose R Botella,
- Nicholas C Carpita,
- Tyrell Carr,
- Jin‐Gui Chen,
- Thomas Ryan Cooke,
- Arwen Frick‐Cheng,
- Erin J Friedman,
- Brandon Fulk,
- Michael G Hahn,
- Kun Jiang,
- Lucia Jorda,
- Lydia Kruppe,
- Chenggang Liu,
- Justine Lorek,
- Maureen C McCann,
- Antonio Molina,
- Etsuko N Moriyama,
- M Shahid Mukhtar,
- Yashwanti Mudgil,
- Sivakumar Pattathil,
- John Schwarz,
- Steven Seta,
- Matthew Tan,
- Ulrike Temp,
- Yuri Trusov,
- Daisuke Urano,
- Bastian Welter,
- Jing Yang,
- Ralph Panstruga,
- Joachim F Uhrig,
- Alan M Jones
Affiliations
- Karsten Klopffleisch
- Botanical Institute, University of Cologne
- Nguyen Phan
- Department of Biology, University of North Carolina at Chapel Hill
- Kelsey Augustin
- Department of Computer Technology and Information Systems, Wayne State College
- Robert S Bayne
- Department of Biology, University of North Carolina at Chapel Hill
- Katherine S Booker
- Department of Biology, University of North Carolina at Chapel Hill
- Jose R Botella
- School of Agriculture and Food Science, University of Queensland
- Nicholas C Carpita
- Department of Botany and Plant Pathology and Bindley Bioscience Center, Purdue University
- Tyrell Carr
- Department of Biology, University of North Carolina at Chapel Hill
- Jin‐Gui Chen
- Oak Ridge National Laboratory, Biosciences Division
- Thomas Ryan Cooke
- Complex Carbohydrate Research Center, University of Georgia
- Arwen Frick‐Cheng
- Department of Biology, University of North Carolina at Chapel Hill
- Erin J Friedman
- Department of Biology, University of North Carolina at Chapel Hill
- Brandon Fulk
- School of Biological Sciences and Center for Plant Science Innovation, University of Nebraska‐Lincoln
- Michael G Hahn
- Complex Carbohydrate Research Center, University of Georgia
- Kun Jiang
- Department of Biology, University of North Carolina at Chapel Hill
- Lucia Jorda
- Centro de Biotecnología y Genomica de Plantas (UPM‐INIA), Universidad Politécnica de Madrid
- Lydia Kruppe
- Botanical Institute, University of Cologne
- Chenggang Liu
- Department of Biology, University of North Carolina at Chapel Hill
- Justine Lorek
- Max‐Planck Institute for Plant Breeding Research
- Maureen C McCann
- Department of Botany and Plant Pathology and Bindley Bioscience Center, Purdue University
- Antonio Molina
- Centro de Biotecnología y Genomica de Plantas (UPM‐INIA), Universidad Politécnica de Madrid
- Etsuko N Moriyama
- School of Biological Sciences and Center for Plant Science Innovation, University of Nebraska‐Lincoln
- M Shahid Mukhtar
- Department of Biology, University of North Carolina at Chapel Hill
- Yashwanti Mudgil
- Department of Biology, University of North Carolina at Chapel Hill
- Sivakumar Pattathil
- Complex Carbohydrate Research Center, University of Georgia
- John Schwarz
- Department of Biostatistics, University of North Carolina at Chapel Hill
- Steven Seta
- Department of Biology, University of North Carolina at Chapel Hill
- Matthew Tan
- Department of Biology, University of North Carolina at Chapel Hill
- Ulrike Temp
- Botanical Institute, University of Cologne
- Yuri Trusov
- School of Agriculture and Food Science, University of Queensland
- Daisuke Urano
- Department of Biology, University of North Carolina at Chapel Hill
- Bastian Welter
- Botanical Institute, University of Cologne
- Jing Yang
- Department of Biology, University of North Carolina at Chapel Hill
- Ralph Panstruga
- Max‐Planck Institute for Plant Breeding Research
- Joachim F Uhrig
- Botanical Institute, University of Cologne
- Alan M Jones
- Department of Biology, University of North Carolina at Chapel Hill
- DOI
- https://doi.org/10.1038/msb.2011.66
- Journal volume & issue
-
Vol. 7,
no. 1
pp. 1 – 7
Abstract
Abstract The heterotrimeric G‐protein complex is minimally composed of Gα, Gβ, and Gγ subunits. In the classic scenario, the G‐protein complex is the nexus in signaling from the plasma membrane, where the heterotrimeric G‐protein associates with heptahelical G‐protein‐coupled receptors (GPCRs), to cytoplasmic target proteins called effectors. Although a number of effectors are known in metazoans and fungi, none of these are predicted to exist in their canonical forms in plants. To identify ab initio plant G‐protein effectors and scaffold proteins, we screened a set of proteins from the G‐protein complex using two‐hybrid complementation in yeast. After deep and exhaustive interrogation, we detected 544 interactions between 434 proteins, of which 68 highly interconnected proteins form the core G‐protein interactome. Within this core, over half of the interactions comprising two‐thirds of the nodes were retested and validated as genuine in planta. Co‐expression analysis in combination with phenotyping of loss‐of‐function mutations in a set of core interactome genes revealed a novel role for G‐proteins in regulating cell wall modification.
Keywords
