Identification of Staphylococcus aureus that Isolated from Cosmetics Products and Detection resistant Genes by used Specialized molecular Markers

Abstract

Background:Cosmetics as well as (Make-up) are used to enhance the appearance or odor of the body and many cosmetics are designed to be placed on the face or hair. Cosmetics is an essential substance of growth and development of many types of micro-organisms because they contain nutrients facilitate the growth of these organisms. Objective: Identification of Staphylococcus aureus by used 16srRNA gene and detection of virulence genes etb , mec A. Patients and Methods: This study includes 100 samples of cosmetic products which are the Compact powder , Foundation , Lipstick and Mascara . Isolated Staphylococcus aureus , identification it and detection of resistant genes by used Polymerase Chain Reaction technique. Results: 35 isolates were identified as Staphylococcus aureus . 4 isolates were identified genetical by 16srRNA gene and 3 isolates detection of resistant genes mecA, etb by used Polymerase Chain Reaction technique. The results of the genetic identification of the 16srRNA using (PCR) , revealed that all S.aureus isolates which were 4 were positive and the results of resistant genes mec A and etb revealed that all S.aureus isolates which were 3 isolates were containing mec A gene depending on the appearance of band asize of(300 base pair) in the agarose gel and all S.aureus isolates were not containing etb gene . Conclusion: It was observed that all S. aureus isolates were resistant for methicillin and that all S.aureus isolates have not Endothelin receptor Type B

Keywords

• Cosmetic productes, Staphylococcus aureus ,Molecular detection , Resistant genes