mSystems (Jun 2021)
MetFish: a Metabolomics Pipeline for Studying Microbial Communities in Chemically Extreme Environments
- Chengdong Xu,
- Sneha P. Couvillion,
- Ryan L. Sontag,
- Nancy G. Isern,
- Yukari Maezato,
- Stephen R. Lindemann,
- Taniya Roy Chowdhury,
- Rui Zhao,
- Beau R. Morton,
- Rosalie K. Chu,
- Ronald J. Moore,
- Janet K. Jansson,
- Vanessa L. Bailey,
- Paula J. Mouser,
- Margaret F. Romine,
- James F. Fredrickson,
- Thomas O. Metz
Affiliations
- Chengdong Xu
- Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
- Sneha P. Couvillion
- ORCiD
- Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
- Ryan L. Sontag
- ORCiD
- Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
- Nancy G. Isern
- Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
- Yukari Maezato
- Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
- Stephen R. Lindemann
- ORCiD
- Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
- Taniya Roy Chowdhury
- ORCiD
- Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
- Rui Zhao
- Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
- Beau R. Morton
- Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
- Rosalie K. Chu
- ORCiD
- Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
- Ronald J. Moore
- ORCiD
- Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
- Janet K. Jansson
- ORCiD
- Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
- Vanessa L. Bailey
- ORCiD
- Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
- Paula J. Mouser
- ORCiD
- Department of Civil and Environmental Engineering, University of New Hampshire, Durham, New Hampshire, USA
- Margaret F. Romine
- ORCiD
- Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
- James F. Fredrickson
- ORCiD
- Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
- Thomas O. Metz
- ORCiD
- Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
- DOI
- https://doi.org/10.1128/mSystems.01058-20
- Journal volume & issue
-
Vol. 6,
no. 3
Abstract
The identification and accurate quantification of metabolites using electrospray ionization-mass spectrometry (ESI-MS) in hypersaline samples is a challenge due to matrix effects. Clean-up and desalting strategies that typically work well for samples with lower salt concentrations are often ineffective in hypersaline samples.
