Analysis of the SC8-0071-014 and sc47-18 loci co-segregated with Ren1 gene in the genotypes of seedless grape varieties

Abstract

Powdery mildew (Erysiphe necator) is one of the most common and economically significant diseases of grapes. The main method of controlling the disease is pesticide treatment. To reduce chemical treatments, it is necessary to select and introduce resistant varieties into production. DNA markers are currently actively used in the study of grape genetic resources. Seedless grape varieties are highly demanded by consumers. Ren1 is one of the known and mapped vine resistance genes to powdery mildew, inherited from V. vinifera; linked DNA markers for this resistance locus are known. A study of 34 seedless grape genotypes was carried out using DNA markers SC8-0071-014 and sc47-18 co-segregated with Ren1. In the studied sample of varieties, 12 types of alleles were identified in the sc47-18 locus and 9 types of alleles in the SC8-0071-014 locus. Target fragments, according to linked marker loci, indicating the presence of the Ren1 resistance gene, were identified in grape variety Lotus (Kriulyanskiy x Yangi Er).