PLoS ONE (Jan 2017)

miRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole.

  • Andrea Russo,
  • Marco Ragusa,
  • Cristina Barbagallo,
  • Antonio Longo,
  • Teresio Avitabile,
  • Maurizio G Uva,
  • Vincenza Bonfiglio,
  • Mario D Toro,
  • Rosario Caltabiano,
  • Cesare Mariotti,
  • Francesco Boscia,
  • Mario Romano,
  • Cinzia Di Pietro,
  • Davide Barbagallo,
  • Michele Purrello,
  • Michele Reibaldi

DOI
https://doi.org/10.1371/journal.pone.0174297
Journal volume & issue
Vol. 12, no. 3
p. e0174297

Abstract

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The aim of the present study was to assess the expression of miRNAs in the Vitreous Humor (VH) of patients with Macular Hole (MH) and Epiretinal Membrane (ERM) compared to a control group.In this prospective, comparative study, 2-ml of VH was extracted from the core of the vitreous chamber in consecutive patients who underwent standard vitrectomy for ERM and MH. RNA was extracted and TaqMan® Low Density Arrays (TLDAs) were used to profile the transcriptome of 754 miRNAs. Results were validated by single TaqMan® assays. Finally, we created a biological network of differentially expressed miRNA targets and their nearest neighbors.Overall 10 eyes with MH, 16 eyes with idiopathic ERM and 6 controls were enrolled in the study. Profiling data identified 5 miRNAs differentially expressed in patients affected by MH and ERM with respect to controls. Four were downregulated (miR-19b, miR-24, miR-155, miR-451) and 1 was downregulated (miR-29a); TaqMan® assays of the VH of patients affected by MH and ERM, with respect to controls, showed that the most differentially expressed were miR-19b (FC -9.13, p:<0.00004), mir-24 (FC -7.52, p:<0.004) and miR-142-3p (FC -5.32, p:<0.011). Our network data showed that deregulation of differentially expressed miRNAs induces an alteration of several pathways associated with genes involved in both MH and ERM.The present study suggests that disregulation of miR-19b, miR-24 and miR-142-3p, might be related to the alterations that characterize patients affected by MH and ERM.