Nature Communications (Dec 2017)

Structure of outer membrane protein G in lipid bilayers

  • Joren S. Retel,
  • Andrew J. Nieuwkoop,
  • Matthias Hiller,
  • Victoria A. Higman,
  • Emeline Barbet-Massin,
  • Jan Stanek,
  • Loren B. Andreas,
  • W. Trent Franks,
  • Barth-Jan van Rossum,
  • Kutti R. Vinothkumar,
  • Lieselotte Handel,
  • Gregorio Giuseppe de Palma,
  • Benjamin Bardiaux,
  • Guido Pintacuda,
  • Lyndon Emsley,
  • Werner Kühlbrandt,
  • Hartmut Oschkinat

DOI
https://doi.org/10.1038/s41467-017-02228-2
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 10

Abstract

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Porins, like OmpG, are embedded in the outer membrane of bacteria and facilitate uptake and secretion of nutrients and ions. Here the authors present a protocol for solid state NMR structure determination of proteins larger than 25 kDa and use it to structurally characterize membrane embedded OmpG.