The characteristics of expression proliferation markers in endometrial hyperplasia

Abstract

Objective. Determination of the mRNA expression genes of cell proliferation and tumor suppressor gene PTEN tumor growth in different types of endometrial hyperplasia and estimate the possibility of their use in clinical practice. Materials and methods. A clinical and laboratory examination and sampling of endometrial tissue in 150 women were obtained. The study group included 103 patients – with endometrial hyperplasia (70 – with a simple, 18 – with a complex, 15 – with atypical), control group – 47 women with morphologically normal endometrium proliferative (n=26) and secretory (n=21) phase. Expression of mRNA of proliferation genes: MKI-67, CCNB1, BIRC5 and PTEN, was performed using RT-PCR. Additionally the expression of Ki-67 and PTEN was determined by immunohistochemistry. Results. The study revealed a reduction mRNA expression of genes MKI67, CCNB1 and BIRC5 in endometrial hyperplasia in comparison with the endometrium of proliferative phase (p

Keywords

• ki-67
• pten
• endometrial hyperplasia
• proliferation
• rt-pcr
• immunohistochemistry
• ki-67
• pten