Spectrphotometric Determination of Buclizine as Hydrochloride Using Various Chromogenic Reagents

Abstract

Simple, accurate and reproducible UV spectrophotometric methods were established for the assay of buclizine (BUCZ) based on the formation of precipitation, charge transfer complex and inner complex formation. Method A involves the precipitation, charge transfer complex formation of BUCZ with tannic acid/ p-N-methyl aminophenol sulphate (PMAP)-Cr(VI), the complex formation with cobalt thiocyanate (CTC) in method B and inner complex formation of BUCZ with sodium nitroprusside hydroxylamine hydrochloride and sodium carbonate in method C were proposed. The optical characteristics such as Beers law limits, molar absorptivity and Sandell’s sensitivity for the methods (A-C) are given. Regression analysis using the method of least squares was made to evaluate the slope(b), intercept(a) and correlation coefficient (r) and standard error of estimation (Se) for each system Determination of BUCZ in bulk form and in pharmaceutical formulations were also incorporated.