Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities

Kendall R. Sanson; Ruth E. Hanna; Mudra Hegde; Katherine F. Donovan; Christine Strand; Meagan E. Sullender; Emma W. Vaimberg; Amy Goodale; David E. Root; Federica Piccioni; John G. Doench

doi:10.1038/s41467-018-07901-8

Nature Communications (Dec 2018)

Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities

Kendall R. Sanson,
Ruth E. Hanna,
Mudra Hegde,
Katherine F. Donovan,
Christine Strand,
Meagan E. Sullender,
Emma W. Vaimberg,
Amy Goodale,
David E. Root,
Federica Piccioni,
John G. Doench

Affiliations

Kendall R. Sanson: Broad Institute of Harvard and MIT
Ruth E. Hanna: Broad Institute of Harvard and MIT
Mudra Hegde: Broad Institute of Harvard and MIT
Katherine F. Donovan: Broad Institute of Harvard and MIT
Christine Strand: Broad Institute of Harvard and MIT
Meagan E. Sullender: Broad Institute of Harvard and MIT
Emma W. Vaimberg: Broad Institute of Harvard and MIT
Amy Goodale: Broad Institute of Harvard and MIT
David E. Root: Broad Institute of Harvard and MIT
Federica Piccioni: Broad Institute of Harvard and MIT
John G. Doench: Broad Institute of Harvard and MIT

DOI: https://doi.org/10.1038/s41467-018-07901-8
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 15

Abstract

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Genome-wide libraries for CRISPR knockout, interference, and activation have allowed the systemic interrogation of gene function. Here, the authors evaluate the Brunello CRISPRko library and introduce Dolcetto and Calabrese for CRISPRi and CRISPRa, respectively.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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