STAR Protocols (Jun 2021)
Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver
- Liliana Sokol,
- Vincent Geldhof,
- Melissa García-Caballero,
- Nadine V. Conchinha,
- Sébastien J. Dumas,
- Elda Meta,
- Laure-Anne Teuwen,
- Koen Veys,
- Rongyuan Chen,
- Lucas Treps,
- Mila Borri,
- Pauline de Zeeuw,
- Kim D. Falkenberg,
- Charlotte Dubois,
- Magdalena Parys,
- Laura P.M.H. de Rooij,
- Jermaine Goveia,
- Katerina Rohlenova,
- Luc Schoonjans,
- Mieke Dewerchin,
- Guy Eelen,
- Xuri Li,
- Joanna Kalucka,
- Peter Carmeliet
Affiliations
- Liliana Sokol
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Vincent Geldhof
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Melissa García-Caballero
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Nadine V. Conchinha
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Sébastien J. Dumas
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Elda Meta
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Laure-Anne Teuwen
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium; Translational Cancer Research Unit, GZA Hospitals Sint-Augustinus, Antwerp 2610, Belgium and Center for Oncological Research, University of Antwerp, Antwerp 2000, Belgium
- Koen Veys
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Rongyuan Chen
- State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, Guangdong 510060, P.R. China
- Lucas Treps
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Mila Borri
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Pauline de Zeeuw
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Kim D. Falkenberg
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Charlotte Dubois
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Magdalena Parys
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Laura P.M.H. de Rooij
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Jermaine Goveia
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Katerina Rohlenova
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Luc Schoonjans
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, Guangdong 510060, P.R. China
- Mieke Dewerchin
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Guy Eelen
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium
- Xuri Li
- State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, Guangdong 510060, P.R. China
- Joanna Kalucka
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium; Department of Biomedicine, Aarhus University, Aarhus 8000, Denmark; Corresponding author
- Peter Carmeliet
- Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, Guangdong 510060, P.R. China; Department of Biomedicine, Aarhus University, Aarhus 8000, Denmark; Corresponding author
- Journal volume & issue
-
Vol. 2,
no. 2
p. 100489
Abstract
Summary: Endothelial cells (ECs) from the small intestine, colon, liver, and heart have distinct phenotypes and functional adaptations that are dependent on their physiological environment. Gut ECs adapt to low oxygen, heart ECs to contractile forces, and liver ECs to low flow rates. Isolating high-purity ECs in sufficient quantities is crucial to study their functions. Here, we describe protocols combining magnetic and fluorescent activated cell sorting for rapid and reproducible EC purification from four adult murine tissues.For complete details on the use and execution of these protocols, please refer to Kalucka et al. (2020).
