Molecules (Jun 2020)
Imunofan—RDKVYR Peptide—Stimulates Skin Cell Proliferation and Promotes Tissue Repair
- Justyna Sawicka,
- Maria Dzierżyńska,
- Anna Wardowska,
- Milena Deptuła,
- Piotr Rogujski,
- Paweł Sosnowski,
- Natalia Filipowicz,
- Alina Mieczkowska,
- Piotr Sass,
- Anna Pawlik,
- Aleksandra Hać,
- Adriana Schumacher,
- Magdalena Gucwa,
- Natalia Karska,
- Jolanta Kamińska,
- Rafał Płatek,
- Jarosław Mazuryk,
- Jacek Zieliński,
- Karolina Kondej,
- Piotr Młynarz,
- Piotr Mucha,
- Piotr Skowron,
- Łukasz Janus,
- Anna Herman-Antosiewicz,
- Paweł Sachadyn,
- Artur Czupryn,
- Arkadiusz Piotrowski,
- Michał Pikuła,
- Sylwia Rodziewicz-Motowidło
Affiliations
- Justyna Sawicka
- Department of Biomedical Chemistry, Faculty of Chemistry, University of Gdańsk, 80-308 Gdańsk, Poland
- Maria Dzierżyńska
- Department of Biomedical Chemistry, Faculty of Chemistry, University of Gdańsk, 80-308 Gdańsk, Poland
- Anna Wardowska
- Laboratory of Tissue Engineering and Regenerative Medicine, Department of Embryology, Medical University of Gdańsk, 80-210 Gdańsk, Poland
- Milena Deptuła
- Laboratory of Tissue Engineering and Regenerative Medicine, Department of Embryology, Medical University of Gdańsk, 80-210 Gdańsk, Poland
- Piotr Rogujski
- Laboratory of Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 02-093 Warsaw, Poland
- Paweł Sosnowski
- Laboratory for Regenerative Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, 80-233 Gdańsk, Poland
- Natalia Filipowicz
- Department of Biology and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Gdańsk, 80-416 Gdańsk, Poland
- Alina Mieczkowska
- Department of Biology and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Gdańsk, 80-416 Gdańsk, Poland
- Piotr Sass
- Laboratory for Regenerative Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, 80-233 Gdańsk, Poland
- Anna Pawlik
- Department of Medical Biology and Genetics, Faculty of Biology, University of Gdańsk, 80-308 Gdańsk, Poland
- Aleksandra Hać
- Department of Medical Biology and Genetics, Faculty of Biology, University of Gdańsk, 80-308 Gdańsk, Poland
- Adriana Schumacher
- Department of Embryology, Medical University of Gdańsk, 80-211 Gdańsk, Poland
- Magdalena Gucwa
- Department of Biology and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Gdańsk, 80-416 Gdańsk, Poland
- Natalia Karska
- Department of Biomedical Chemistry, Faculty of Chemistry, University of Gdańsk, 80-308 Gdańsk, Poland
- Jolanta Kamińska
- Laboratory for Regenerative Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, 80-233 Gdańsk, Poland
- Rafał Płatek
- Laboratory of Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 02-093 Warsaw, Poland
- Jarosław Mazuryk
- Laboratory of Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 02-093 Warsaw, Poland
- Jacek Zieliński
- Department of Surgical Oncology, Medical University of Gdańsk, 80-210 Gdańsk, Poland
- Karolina Kondej
- Department of Plastic Surgery, Medical University of Gdańsk, 80-210 Gdańsk, Poland
- Piotr Młynarz
- Department of Bioorganic Chemistry, Wrocław University of Technology, 50-370 Wrocław, Poland
- Piotr Mucha
- Department of Molecular Biochemistry, Faculty of Chemistry, University of Gdańsk80-308 Gdańsk, Poland
- Piotr Skowron
- Department of Molecular Biotechnology, Faculty of Chemistry, University of Gdańsk, 80-308 Gdańsk, Poland
- Łukasz Janus
- MedVentures Company, 60-141 Poznań, Poland
- Anna Herman-Antosiewicz
- Department of Medical Biology and Genetics, Faculty of Biology, University of Gdańsk, 80-308 Gdańsk, Poland
- Paweł Sachadyn
- Laboratory for Regenerative Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, 80-233 Gdańsk, Poland
- Artur Czupryn
- Laboratory of Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 02-093 Warsaw, Poland
- Arkadiusz Piotrowski
- Department of Biology and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Gdańsk, 80-416 Gdańsk, Poland
- Michał Pikuła
- Laboratory of Tissue Engineering and Regenerative Medicine, Department of Embryology, Medical University of Gdańsk, 80-210 Gdańsk, Poland
- Sylwia Rodziewicz-Motowidło
- Department of Biomedical Chemistry, Faculty of Chemistry, University of Gdańsk, 80-308 Gdańsk, Poland
- DOI
- https://doi.org/10.3390/molecules25122884
- Journal volume & issue
-
Vol. 25,
no. 12
p. 2884
Abstract
Regeneration and wound healing are vital to tissue homeostasis and organism survival. One of the biggest challenges of today’s science and medicine is finding methods and factors to stimulate these processes in the human body. Effective solutions to promote regenerative responses will accelerate advances in tissue engineering, regenerative medicine, transplantology, and a number of other clinical specialties. In this study, we assessed the potential efficacy of a synthetic hexapeptide, RDKVYR, for the stimulation of tissue repair and wound healing. The hexapeptide is marketed under the name “Imunofan” (IM) as an immunostimulant. IM displayed stability in aqueous solutions, while in plasma it was rapidly bound by albumins. Structural analyses demonstrated the conformational flexibility of the peptide. Tests in human fibroblast and keratinocyte cell lines showed that IM exerted a statistically significant (p p p < 0.05) in fibroblasts and activation of genes involved in immune responses, migration, and chemotaxis in adipose-derived stem cells derived from surgery donors. Experiments in a model of ear pinna injury in mice indicated that IM moderately promoted tissue repair (8% in BALB/c and 36% in C57BL/6 in comparison to control).
Keywords
