An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA

Lapatrada Taemaitree; Arun Shivalingam; Afaf H. El-Sagheer; Tom Brown

doi:10.1038/s41467-019-09600-4

Nature Communications (Apr 2019)

An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA

Lapatrada Taemaitree,
Arun Shivalingam,
Afaf H. El-Sagheer,
Tom Brown

Affiliations

Lapatrada Taemaitree: Department of Chemistry, University of Oxford, Chemistry Research Laboratory
Arun Shivalingam: Department of Chemistry, University of Oxford, Chemistry Research Laboratory
Afaf H. El-Sagheer: Department of Chemistry, University of Oxford, Chemistry Research Laboratory
Tom Brown: Department of Chemistry, University of Oxford, Chemistry Research Laboratory

DOI: https://doi.org/10.1038/s41467-019-09600-4
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 8

Abstract

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For CRISPR-Cas9 genome editing, Cas9 protein is guided to its target by single guide (sg) RNA. Here, the authors synthesised sgRNAs via convergent ‘click’ ligation of variable 20-mer RNAs that target the genome and a Cas9-binding 79-mer chimeric RNA/2´-OMe RNA of fixed sequence in a single tube.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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