Effects of Different Carbohydrate Content Diet on Gut Microbiota and Aortic Calcification in Diabetic Mice

Abstract

Xinyi Shen,1,2 Ge Guo,1,2 Guoquan Feng,3 Zhongqun Wang1,2 1Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 212000, People’s Republic of China; 2Institute of Cardiovascular Diseases, Jiangsu University, Zhenjiang, 212001, People’s Republic of China; 3Imaging Department, Affiliated Hospital of Jiangsu University, Zhenjiang, 212000, People’s Republic of ChinaCorrespondence: Zhongqun Wang, Email [email protected]: Vascular calcification is a major cause of cardiovascular accidents in patients with type 2 diabetes mellitus. This study aimed to investigate the impact of carbohydrates on gut microbiota and aortic calcification in diabetic ApoE−/− mice.Methods: The diabetic ApoE−/− mice were randomly divided into 4 groups: ketogenic diet group, low carbohydrate diet group, medium carbohydrate diet group, and high carbohydrate diet group. The mice were fed continuously for 6 months, with blood glucose, blood ketone and body weight monitored monthly. Lipid metabolism indicators and inflammatory factors were detected using ELISA. The intestinal barrier, atherosclerotic lesion areas, and vascular calcifications were analyzed based on their morphology. Gut microbiota was analyzed using 16S rRNA genes.Results: We found that ketogenic diet played some roles improving glucose, lipid metabolism, and inflammation. Ketogenic diet could improve the intestinal barrier to some extent and increase intestinal bacteria. Compared to the other three groups, the relative abundance of genus Allobaculum, species Blautia producta and Clostridium Ramosum in the ketogenic diet group was significantly increased (P < 0.05), which has protective effects in diabetic ApoE−/− mice.Conclusion: Ketogenic diet could delay the onset of aortic atherosclerosis, aortic calcification and improve intestinal barrier function in diabetic ApoE−/− mice. Keywords: diabetes mellitus, atherosclerosis, vascular calcification, gut microbiota, low-carb diet, ketogenic diet, inflammation

Keywords

• diabetes mellitus
• atherosclerosis
• vascular calcification
• gut microbiota
• low-carb diet
• ketogenic diet
• inflammation