Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia

Abstract

Giardia lamblia is a human protozoan parasite with two diploid nuclei, which makes complete knock-out of a gene of interest challenging. Here the authors use a Cre/loxP-based approach to knock-out cyst-wall protein 1 (cwp1) and show that CWP1 is essential for cyst-wall biosynthesis.