Protective Effects of Ellagic Acid on Testicular Ischemia-Reperfusion Injury in Rats

Abstract

Objective:This study aimed to investigate the protective effects of ellagic acid on testicular ischemia/reperfusion injury in rats.Materials and Methods:Twenty-one Sprague-Dawley rats were randomly divided into three groups: sham, ischemia/reperfusion (I/R), I/R + ellagic acid (EA). All animals underwent left scrotal exploration. In all groups except the sham group, the left testes were rotated 720 degrees clockwise for 3 h and 3 h reperfusion. 10 mg/kg ellagic acid was administered intraperitoneally to the I/R+E group before reperfusion. Then, the left orchiectomy was performed. The testes underwent biochemical and histological examination.Results:There was a significant difference between the sham and the I/R, I/R+EA groups according to the Cosentino system (p<0.001, p=0.036), and there was no difference between the I/R and IR+EA groups (p=0.319). A significant difference was found between sham and I/R groups according to Johnsen spermatogenesis score (p<0.001), but there was no significant difference between sham and I/R+EA groups (p=0.063). Superoxide dismutase, catalase, malondialdehyde, total oxidant status values were statistically different between I/R and I/R+EA groups (p=0.001, 0.002, 0.002, 0.001 respectively).Conclusion:Ellagic acid has a protective effect against testicular ischemia/reperfusion injury in rats.

Keywords

• ellagic acid
• ischemia/reperfusion injury
• testis