Rab27a promotes degradation of West Nile virus E protein in the lysosome

Shintaro Kobayashi; Seira Kawai; Yukine Fukuda; Haruto Eguchi; Keisuke Maezono; Passawat Thammahakin; Hirofumi Sawa; Hiroaki Kariwa

iScience (Apr 2024)

Rab27a promotes degradation of West Nile virus E protein in the lysosome

Shintaro Kobayashi,
Seira Kawai,
Yukine Fukuda,
Haruto Eguchi,
Keisuke Maezono,
Passawat Thammahakin,
Hirofumi Sawa,
Hiroaki Kariwa

Affiliations

Shintaro Kobayashi: Laboratory of Public Health, Faculty of Veterinary medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan; Hokkaido University, Institute for Vaccine Research and Development (HU-IVReD), Sapporo, Hokkaido 001-0021, Japan; Corresponding author
Seira Kawai: Laboratory of Public Health, Faculty of Veterinary medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan
Yukine Fukuda: Laboratory of Public Health, Faculty of Veterinary medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan
Haruto Eguchi: Laboratory of Public Health, Faculty of Veterinary medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan
Keisuke Maezono: Laboratory of Public Health, Faculty of Veterinary medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan
Passawat Thammahakin: Laboratory of Public Health, Faculty of Veterinary medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan
Hirofumi Sawa: Hokkaido University, Institute for Vaccine Research and Development (HU-IVReD), Sapporo, Hokkaido 001-0021, Japan; Division of Molecular Pathobiology, International Institute for Zoonosis Control, N20, W10, Kita-ku, Sapporo, Hokkaido 001-0020, Japan; One Health Research Center, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan; International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan; Global Virus Network, Baltimore, MD 21201, USA
Hiroaki Kariwa: Laboratory of Public Health, Faculty of Veterinary medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan

Journal volume & issue: Vol. 27, no. 4
p. 109539

Abstract

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Summary: Rab27a, a Rab family small GTPases, plays an important role in the trafficking and secretion of the intracellular proteins and has been reported to promote various viral multiplication. However, whether Rab27a is involved in West Nile virus (WNV) multiplication is unknown. This study examined the ability of Rab27a to suppress WNV multiplication. The inhibition of Rab27a expression increased viral multiplication and the intracellular levels of WNV structural proteins, E and prM proteins. Rab27a partially colocalized with E protein, mainly in the perinuclear region, while inhibition of Rab27a expression resulted in diffuse subcellular localization of E protein. In addition, some of the perinuclear E protein colocalized with the lysosomal marker LAMP1, and inhibition of lysosomal acidification increased intracellular levels of Rab27a and E proteins. These observations suggested that Rab27a inhibits WNV multiplication by inducing the degradation of viral protein in lysosomes.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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