Journal of Krishna Institute of Medical Sciences University (Jul 2020)
Structural Modeling and Validation of Growth/Differentiation Factor 15 [NP_004855] Associated with Pregnancy Complication- Hyperemesis Gravidarum
Abstract
Background: Hyperemesis Gravidarum (HG) is a common pregnancy complication that occurs in 0.3–2% of pregnancies. Growth/Differentiation Factor (GDF) 15 serum levels are abnormally high in patients associated with HG. In silico analysis provides information about structure and function of GDF15. Aim and Objectives: The aim of this study was to enlist biochemical and functional properties of GDF15 protein and determine its three-dimensional structure, as GDF15 is known to be associated with risk of HG. Material and Methods: The PDB file of GDF15 [NP_004855] was created by RaptorX structure prediction server. The UCLA-DOE server was used to visual analysis of crystal structure of protein. The validation for structure models was performed by using PROCHECK. Model quality estimates were based on the QMEAN and ProSA. Results: The model showed good stereo-chemical property in terms of G-factor value -0.64, that indicates geometry of model corresponds to probability conformation with 95% residue in the favored region of Ramachandran plot, showing high accuracy of model prediction. The Zscore of -4.04 predicted by ProSA represents the good quality of the model. The energy plot shows the local model quality by plotting knowledge-based energies as a function of amino acid sequence position. Conclusion: The generated model could be supportive to understand the structure and functional characteristics of Homo sapiens growth/differentiation factor 15 [NP_004855]. As abnormal high serum levels of GDF15 were observe in patients associated with HG. Therefore, the structure model of GDF15 [NP_004855] is useful to understand its role in development of HG. In Silico docking study could be explain the molecular association of GDF15 [NP_004855] with HG and new drug designing, for that structure model is very useful.
