Nature Communications (Aug 2022)
Native metabolomics identifies the rivulariapeptolide family of protease inhibitors
- Raphael Reher,
- Allegra T. Aron,
- Pavla Fajtová,
- Paolo Stincone,
- Berenike Wagner,
- Alicia I. Pérez-Lorente,
- Chenxi Liu,
- Ido Y. Ben Shalom,
- Wout Bittremieux,
- Mingxun Wang,
- Kyowon Jeong,
- Marie L. Matos-Hernandez,
- Kelsey L. Alexander,
- Eduardo J. Caro-Diaz,
- C. Benjamin Naman,
- J. H. William Scanlan,
- Phil M. M. Hochban,
- Wibke E. Diederich,
- Carlos Molina-Santiago,
- Diego Romero,
- Khaled A. Selim,
- Peter Sass,
- Heike Brötz-Oesterhelt,
- Chambers C. Hughes,
- Pieter C. Dorrestein,
- Anthony J. O’Donoghue,
- William H. Gerwick,
- Daniel Petras
Affiliations
- Raphael Reher
- Scripps Institution of Oceanography, University of California San Diego
- Allegra T. Aron
- Skaggs School of Pharmacy and Pharmaceutical Science, University of California San Diego
- Pavla Fajtová
- Skaggs School of Pharmacy and Pharmaceutical Science, University of California San Diego
- Paolo Stincone
- Cluster of Excellence “Controlling Microbes to Fight Infections” (CMFI), University of Tuebingen
- Berenike Wagner
- Cluster of Excellence “Controlling Microbes to Fight Infections” (CMFI), University of Tuebingen
- Alicia I. Pérez-Lorente
- Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora,” Consejo Superior de Investigaciones Científicas, Departamento de Microbiología, Universidad de Málaga
- Chenxi Liu
- Skaggs School of Pharmacy and Pharmaceutical Science, University of California San Diego
- Ido Y. Ben Shalom
- Skaggs School of Pharmacy and Pharmaceutical Science, University of California San Diego
- Wout Bittremieux
- Skaggs School of Pharmacy and Pharmaceutical Science, University of California San Diego
- Mingxun Wang
- Skaggs School of Pharmacy and Pharmaceutical Science, University of California San Diego
- Kyowon Jeong
- Applied Bioinformatics, Computer Science Department, University of Tuebingen
- Marie L. Matos-Hernandez
- Department of Pharmaceutical Sciences, School of Pharmacy, University of Puerto Rico - Medical Sciences Campus
- Kelsey L. Alexander
- Scripps Institution of Oceanography, University of California San Diego
- Eduardo J. Caro-Diaz
- Department of Pharmaceutical Sciences, School of Pharmacy, University of Puerto Rico - Medical Sciences Campus
- C. Benjamin Naman
- Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Department of Marine Pharmacy, College of Food and Pharmaceutical Sciences, Ningbo University
- J. H. William Scanlan
- Department of Pharmaceutical Chemistry and Center for Tumor Biology and Immunology (ZTI), University of Marburg
- Phil M. M. Hochban
- Department of Pharmaceutical Chemistry and Center for Tumor Biology and Immunology (ZTI), University of Marburg
- Wibke E. Diederich
- Department of Pharmaceutical Chemistry and Center for Tumor Biology and Immunology (ZTI), University of Marburg
- Carlos Molina-Santiago
- Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora,” Consejo Superior de Investigaciones Científicas, Departamento de Microbiología, Universidad de Málaga
- Diego Romero
- Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora,” Consejo Superior de Investigaciones Científicas, Departamento de Microbiología, Universidad de Málaga
- Khaled A. Selim
- Cluster of Excellence “Controlling Microbes to Fight Infections” (CMFI), University of Tuebingen
- Peter Sass
- Cluster of Excellence “Controlling Microbes to Fight Infections” (CMFI), University of Tuebingen
- Heike Brötz-Oesterhelt
- Cluster of Excellence “Controlling Microbes to Fight Infections” (CMFI), University of Tuebingen
- Chambers C. Hughes
- Cluster of Excellence “Controlling Microbes to Fight Infections” (CMFI), University of Tuebingen
- Pieter C. Dorrestein
- Skaggs School of Pharmacy and Pharmaceutical Science, University of California San Diego
- Anthony J. O’Donoghue
- Skaggs School of Pharmacy and Pharmaceutical Science, University of California San Diego
- William H. Gerwick
- Scripps Institution of Oceanography, University of California San Diego
- Daniel Petras
- Scripps Institution of Oceanography, University of California San Diego
- DOI
- https://doi.org/10.1038/s41467-022-32016-6
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 12
Abstract
Bioactivity-guided isolation of specialized metabolites is an iterative process. Here, the authors demonstrate a native metabolomics approach that allows for fast screening of complex metabolite extracts against a protein of interest and simultaneous structure annotation.
