PLoS ONE (Jan 2022)

Serum miR-34a-5p and miR-199a-3p as new biomarkers of neonatal sepsis.

  • Omayma O Abdelaleem,
  • Shereen Rashad Mohammed,
  • Hassan S El Sayed,
  • Sherin Khamis Hussein,
  • Doaa Y Ali,
  • Mostafa Y Abdelwahed,
  • Sylvana N Gaber,
  • Nada F Hemeda,
  • Rehab G Abd El-Hmid

DOI
https://doi.org/10.1371/journal.pone.0262339
Journal volume & issue
Vol. 17, no. 1
p. e0262339

Abstract

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BackgroundNeonatal sepsis is a serious condition. Recent clinical studies have indicated that microRNAs (miRNAs) are key players in the pathogenesis of sepsis, which could be used as biomarkers for this condition.Patients and methodsA total of 90 neonates with sepsis and 90 healthy neonates were enrolled in this study. qRT-PCR was performed to measure the expression levels of serum miR-34a-5p and miR-199a-3p.ResultsmiR-34a-5p and miR-199a-3p serum levels were significantly reduced in neonates with sepsis compared with those in healthy neonates (P = 0.006 and P = 0.001, respectively). Significant correlations of miR-34a-5p and miR-199a-3p with each of TLC, RDW, RBS, and C-reactive protein (CRP) as well as SNAPII were observed, indicating their associations with the severity of neonatal sepsis.ConclusionmiR-34a-5p and miR-199a-3p may be useful as novel biomarkers in neonatal sepsis and may provide a new direction for its treatment.