Frontiers in Immunology (Oct 2023)

Oral exposure to Staphylococcus aureus enterotoxin B could promote the Ovalbumin-induced food allergy by enhancing the activation of DCs and T cells

  • Jin Yuan,
  • Jin Yuan,
  • Jin Yuan,
  • Ping Tong,
  • Xuanyi Meng,
  • Yong Wu,
  • Xin Li,
  • Xin Li,
  • Jinyan Gao,
  • Hongbing Chen,
  • Hongbing Chen

DOI
https://doi.org/10.3389/fimmu.2023.1250458
Journal volume & issue
Vol. 14

Abstract

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IntroductionRecent work highlighted the importance of environmental contaminants in the development of allergic diseases.MethodsThe intestinal mucosal barrier, Th (helper T) cells, DCs (dendritic cells), and intestinal flora were analyzed with flow cytometry, RNA-seq, and 16s sequencing in the present study to demonstrate whether the exposure of enterotoxins like Staphylococcus aureus enterotoxin B (SEB) in allergens could promote the development of food allergy.Results and discussionWe found that co-exposure to SEB and Ovalbumin (OVA) could impair the intestinal barrier, imbalance the intestinal Th immune, and cause the decline of intestinal flora diversity in OVA-sensitized mice. Moreover, with the co-stimulation of SEB, the transport of OVA was enhanced in the Caco-2 cell monolayer, the uptake and presentation of OVA were promoted in the bone marrow dendritic cells (BMDCs), and Th cell differentiation was also enhanced. In summary, co-exposure to SEB in allergens should be considered a food allergy risk factor.

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