Spectroscopic characterization of humic and fulvic acids in soil aggregates, Brazil

Abstract

Agricultural management methods affect organic compounds in the soil. The aim of this study was to characterize humic and fulvic acids using infrared spectroscopy and nuclear magnetic resonance (NMR) in aggregates collected from areas under different crop and soil management systems. Soil samples were collected at depths of 0.00–0.10 m in a trial set up in 1989 with four management systems (no-till, no-till with chiseling every three years, disk harrowing and heavy disking) under two production systems (succession and rotation). In the laboratory, the soil was sieved and separated into two fractions: particle size of 2 mm or more, and less than 2 mm. From each of the samples an aliquot was extracted and the humic and fulvic acids purified for FTIR, UV-VIS and NMR-1H analysis. The results obtained showed that the structural characterization of the humic and fulvic acid fractions were linked to organic matter left on the soil surface, especially where the agricultural system involved species diversity. Aggregates ≥2 mm produce higher NMR, UV-VIS and FTIR spectral magnitudes in aromatic and aliphatic structures than those <2 mm. Aggregates collected from soil under no-till retained their aromatic and aliphatic chemical structures, resulting in higher spectral amplitude.

Keywords

• Agriculture
• Soil science
• Humic substances
• Crop science