Revista da Sociedade Brasileira de Medicina Tropical ()

Clonal relation and antimicrobial resistance pattern of extended-spectrum β-lactamase- and AmpC β-lactamase-producing Enterobacter spp. isolated from different clinical samples in Tehran, Iran

  • Roya Ghanavati,
  • Mohammad Emaneini,
  • Davood Kalantar-Neyestanaki,
  • Azin Sattari Maraji,
  • Mosayyeb Dalvand,
  • Reza Beigverdi,
  • Fereshteh Jabalameli

DOI
https://doi.org/10.1590/0037-8682-0227-2017
Journal volume & issue
Vol. 51, no. 1
pp. 88 – 93

Abstract

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Abstract INTRODUCTION: Here, we determined the genes encoding antibiotic resistance enzymes and virulence factors and evaluated the genetic relationship between Enterobacter spp. isolated from different clinical samples. METHODS: A total of 57 clinical isolates of Enterobacter spp. were tested for the production of extended-spectrum β-lactamases (ESBLs), carbapenemase, and AmpC using phenotypic and genotypic methods. RESULTS: The most common ESBLs and AmpC β-lactamases were bla TEM (63.3%) and bla EBC (57.7%), respectively. The most prevalent virulence gene was rpos (87.7%). The random amplified polymorphic DNA (RAPD) patterns of strains were genetically unrelated. CONCLUSIONS: RAPD polymerase chain reaction analysis revealed high genetic diversity among isolates.

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