Bioactive 2-(Methyldithio)Pyridine-3-Carbonitrile from Persian Shallot (Allium stipitatum Regel.) Exerts Broad-Spectrum Antimicrobial Activity

Arunkumar Karunanidhi; Ehsanollah Ghaznavi-Rad; Jayakayatri Jeevajothi Nathan; Narcisse Joseph; Sridevi Chigurupati; Fazlin Mohd Fauzi; Mallikarjuna  Rao Pichika; Rukman  Awang Hamat; Leslie  Than Thian Lung; Alex van Belkum; Vasanthakumari Neela

doi:10.3390/molecules24061003

Molecules (Mar 2019)

Bioactive 2-(Methyldithio)Pyridine-3-Carbonitrile from Persian Shallot (Allium stipitatum Regel.) Exerts Broad-Spectrum Antimicrobial Activity

Arunkumar Karunanidhi,
Ehsanollah Ghaznavi-Rad,
Jayakayatri Jeevajothi Nathan,
Narcisse Joseph,
Sridevi Chigurupati,
Fazlin Mohd Fauzi,
Mallikarjuna Rao Pichika,
Rukman Awang Hamat,
Leslie Than Thian Lung,
Alex van Belkum,
Vasanthakumari Neela

Affiliations

Arunkumar Karunanidhi: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia
Ehsanollah Ghaznavi-Rad: Department of Microbiology and Immunology, Faculty of Medicine, Arak University of Medical Sciences, Basij Square, Arak 38481-7-6941, Iran
Jayakayatri Jeevajothi Nathan: School of Medicine, Taylor’s University, Lakeside Campus, No. 1, Jalan Taylor’s, Subang Jaya 47500, Selangor Darul Ehsan, Malaysia
Narcisse Joseph: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia
Sridevi Chigurupati: Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraidah 52571, Kingdom of Saudi Arabia
Fazlin Mohd Fauzi: Department of Pharmacology and Chemistry, Faculty of Pharmacy, Universiti Teknologi MARA, Bandar Puncak Alam 42300, Selangor Darul Ehsan, Malaysia
Mallikarjuna Rao Pichika: Department of Pharmaceutical Chemistry, School of Pharmacy, International Medical University, 126 Jalan Bukit Jalil, Kuala Lumpur 57000, Malaysia
Rukman Awang Hamat: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia
Leslie Than Thian Lung: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia
Alex van Belkum: R & D Microbiology, BioMerieux, 3 route de Port Michaud, 38390 La Balme-les-Grottes, France
Vasanthakumari Neela: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia

DOI: https://doi.org/10.3390/molecules24061003
Journal volume & issue: Vol. 24, no. 6
p. 1003

Abstract

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Antibiotic resistance is a problem that continues to challenge the healthcare sector, especially in clinically significant pathogens like methicillin-resistant Staphylococcus aureus (MRSA). Herein is described the isolation and structure elucidation of a bioactive compound from Allium stipitatum with antimicrobial activity. Crude Allium stipitatum dichloromethane extract (ASDE) was subjected to systematic purification by chromatographic procedures to afford various bioactive fractions. A fraction that exhibited anti-MRSA activity (4 µg·mL−1) was further characterized to determine the structure. The structure of the compound was elucidated as 2-(methyldithio)pyridine-3-carbonitrile (2-Medpy-3-CN). The 2-Medpy-3-CN compound, which was screened for antimicrobial activity, exhibited minimum inhibitory concentrations (MICs) in the range of 0.5 to >64 µg·mL−1 for tested bacterial species and 0.25 to 2 µg·mL−1 for Candida spp. Further studies are important to confirm the drug target and mechanism of action.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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