Diversity of endophytes bacteria isolated from tree neem and its inhibitory activity against Colletotrichum gloesporioides cause the anthracnose of the yam in the Department of Sucre

Abstract

The present study as had objective, test in vitro the antifungal endophytes bacteria activity present on leaves and seeds of Neem trees, found into the Sincelejo local area. The tissue samples were recollected randomly. The recollected tissues were superficially sanitized, subsequently, the isolation, counting and distinction of morphotypes of endophytes bacteria were carried out by the Serial dilution technique through the agar plate. Each isolated morphotypes was used to assess in vitro the inhibitory activity of endophytes bacteria cells against the growth of C. Gloeosporioides fungus. The activity of the endophytes bacteria against the fungus was determined by inhibition index. The results of the factorial analysis established differences between the amount of bacteria present in leaves and seeds, with higher values in leaves (1,8x1010) and with lower values in seeds (1,2x109). The in vitro assay showed inhibitory activity of endophytic bacteria on fungal mycelial growth, the results of the identification with API20E kit confirm with 99,8% identity of the bacteria species, Pseudomona aeuroginosa, Constituting an isolated endophytic bacteria of the neem tree tissues With inhibitory activity against the mycelial growth of C fungus. Gloeosporioides, causing the disease known as anthracnose of Yam crop in the Sucre department.

Keywords

• Microorganismos endófitos
• hongos fitopatógenos
• tejidos vegetales.