Nature Communications (Sep 2018)
Robust single-cell DNA methylome profiling with snmC-seq2
- Chongyuan Luo,
- Angeline Rivkin,
- Jingtian Zhou,
- Justin P. Sandoval,
- Laurie Kurihara,
- Jacinta Lucero,
- Rosa Castanon,
- Joseph R. Nery,
- António Pinto-Duarte,
- Brian Bui,
- Conor Fitzpatrick,
- Carolyn O’Connor,
- Seth Ruga,
- Marc E. Van Eden,
- David A. Davis,
- Deborah C. Mash,
- M. Margarita Behrens,
- Joseph R. Ecker
Affiliations
- Chongyuan Luo
- Genomic Analysis Laboratory, The Salk Institute for Biological Studies
- Angeline Rivkin
- Genomic Analysis Laboratory, The Salk Institute for Biological Studies
- Jingtian Zhou
- Genomic Analysis Laboratory, The Salk Institute for Biological Studies
- Justin P. Sandoval
- Genomic Analysis Laboratory, The Salk Institute for Biological Studies
- Laurie Kurihara
- Swift Biosciences Inc.
- Jacinta Lucero
- Computational Neurobiology Laboratory, The Salk Institute for Biological Studies
- Rosa Castanon
- Genomic Analysis Laboratory, The Salk Institute for Biological Studies
- Joseph R. Nery
- Genomic Analysis Laboratory, The Salk Institute for Biological Studies
- António Pinto-Duarte
- Computational Neurobiology Laboratory, The Salk Institute for Biological Studies
- Brian Bui
- Computational Neurobiology Laboratory, The Salk Institute for Biological Studies
- Conor Fitzpatrick
- Flow Cytometry Core Facility, The Salk Institute for Biological Studies
- Carolyn O’Connor
- Flow Cytometry Core Facility, The Salk Institute for Biological Studies
- Seth Ruga
- Zymo Research Corporation
- Marc E. Van Eden
- Zymo Research Corporation
- David A. Davis
- Department of Neurology, Miller School of Medicine, University of Miami
- Deborah C. Mash
- Department of Neurology, Miller School of Medicine, University of Miami
- M. Margarita Behrens
- Computational Neurobiology Laboratory, The Salk Institute for Biological Studies
- Joseph R. Ecker
- Genomic Analysis Laboratory, The Salk Institute for Biological Studies
- DOI
- https://doi.org/10.1038/s41467-018-06355-2
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 6
Abstract
Single-cell DNA methylome profiling allows the study of epigenomic heterogeneity in tissues but has been impeded by library quality. Here the authors demonstrate snmC-seq2 which improves mapping, throughput and library complexity.
