The biological activity of subspecies Trichoderma harzianum against Fusarium oxysporum, the causative agent of fusarium wilt cucumber in vitro

Abstract

The study of the effect of the strains of the fungus Trichoderma: Trichoderma atrobrunneum VKPM F-1434, Trichoderma harzianum 5/14, Trichoderma Lixii T4/14 on the number of micromycetes populations of the pathogenic fungi Fusarium oxysporum isolate B/14, Fusarium oxysporum isolate MOS509, Fysarium oxysporum isolate IMI58289 in vitro. It was found that the Trichoderma atrobrunneum fungus strain VKPM F-1434 showed the highest degree of inhibition on 10 days of cultivation with phytopathogenic microorganisms, which was 100 %. The study revealed that all Trichoderma species are capable of producing lytic enzymes. Trichoderma atrobrunneum strain VKPM F-1434 exhibits strong lipase and chitinase activity and average proteinase activity. In addition, Trichoderma atrobrunneum strain VKPM F-1434 has a growth-promoting ability, which was reflected in the germination of seeds of cucumber “German F1”. The maximum values of indicators of germination energy were noted – 98.4 % and germination – 100 %.