Application of ARID1A to murine formalin-fixed paraffin embedded tissue using immunohistochemistry [v3; ref status: indexed, http://f1000r.es/5ho]

Will Howat; Jodi Miller; Ioannis Gounaris

doi:10.12688/f1000research.5514.3

F1000Research (Jun 2015)

Application of ARID1A to murine formalin-fixed paraffin embedded tissue using immunohistochemistry [v3; ref status: indexed, http://f1000r.es/5ho]

Will Howat,
Jodi Miller,
Ioannis Gounaris

Affiliations

Will Howat: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, CB2 0RE, UK
Jodi Miller: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, CB2 0RE, UK
Ioannis Gounaris: Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, CB2 0RE, UK

DOI: https://doi.org/10.12688/f1000research.5514.3
Journal volume & issue: Vol. 3

Abstract

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ARID1A is a known suppressor of tumour formation and the Human Protein Atlas antibody HPA005456 has been demonstrated in previous literature to stain tumour tissue by immunohistochemistry (IHC) in formalin-fixed paraffin embedded human tissue and human cell lines. This article details the validation of this antibody for immunohistochemistry of formalin-fixed paraffin embedded murine tissue using a Leica BondMax immunostainer. Using Western blot and IHC on murine wild-type and knockout tissue we have demonstrated that this antibody to ARID1A correctly stains murine tissue by immunohistochemistry.

Immunological Biomarkers

Published in F1000Research

ISSN: 2046-1402 (Online)
Publisher: F1000 Research Ltd
Country of publisher: United Kingdom
LCC subjects: Medicine; Science
Website: https://f1000research.com

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