Lactiplantibacillus plantarum-Derived Biosurfactant Attenuates Quorum Sensing-Mediated Virulence and Biofilm Formation in Pseudomonas aeruginosa and Chromobacterium violaceum

Mitesh Patel; Arif Jamal Siddiqui; Syed Amir Ashraf; Malvi Surti; Amir Mahgoub Awadelkareem; Mejdi Snoussi; Walid Sabri Hamadou; Fevzi Bardakci; Arshad Jamal; Sadaf Jahan; Manojkumar Sachidanandan; Mohd Adnan

doi:10.3390/microorganisms10051026

Microorganisms (May 2022)

Lactiplantibacillus plantarum-Derived Biosurfactant Attenuates Quorum Sensing-Mediated Virulence and Biofilm Formation in Pseudomonas aeruginosa and Chromobacterium violaceum

Mitesh Patel,
Arif Jamal Siddiqui,
Syed Amir Ashraf,
Malvi Surti,
Amir Mahgoub Awadelkareem,
Mejdi Snoussi,
Walid Sabri Hamadou,
Fevzi Bardakci,
Arshad Jamal,
Sadaf Jahan,
Manojkumar Sachidanandan,
Mohd Adnan

Affiliations

Mitesh Patel: Department of Biotechnology, Parul Institute of Applied Sciences and Centre of Research for Development, Parul University, Vadodara 391760, India
Arif Jamal Siddiqui: Department of Biology, College of Science, University of Hail, Hail P.O. Box 2440, Saudi Arabia
Syed Amir Ashraf: Department of Clinical Nutrition, College of Applied Medial Sciences, University of Hail, Hail P.O. Box 2440, Saudi Arabia
Malvi Surti: Bapalal Vaidya Botanical Research Centre, Department of Biosciences, Veer Narmad South Gujarat University, Surat 395007, India
Amir Mahgoub Awadelkareem: Department of Clinical Nutrition, College of Applied Medial Sciences, University of Hail, Hail P.O. Box 2440, Saudi Arabia
Mejdi Snoussi: Department of Biology, College of Science, University of Hail, Hail P.O. Box 2440, Saudi Arabia
Walid Sabri Hamadou: Department of Biology, College of Science, University of Hail, Hail P.O. Box 2440, Saudi Arabia
Fevzi Bardakci: Department of Biology, College of Science, University of Hail, Hail P.O. Box 2440, Saudi Arabia
Arshad Jamal: Department of Biology, College of Science, University of Hail, Hail P.O. Box 2440, Saudi Arabia
Sadaf Jahan: Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al-Majmaah 11952, Saudi Arabia
Manojkumar Sachidanandan: Department of Oral Radiology, College of Dentistry, University of Hail, Hail P.O. Box 2440, Saudi Arabia
Mohd Adnan: Department of Biology, College of Science, University of Hail, Hail P.O. Box 2440, Saudi Arabia

DOI: https://doi.org/10.3390/microorganisms10051026
Journal volume & issue: Vol. 10, no. 5
p. 1026

Abstract

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Quorum sensing (QS) controls the expression of diverse biological traits in bacteria, including virulence factors. Any natural bioactive compound that disables the QS system is being considered as a potential strategy to prevent bacterial infection. Various biological activities of biosurfactants have been observed, including anti-QS effects. In the present study, we investigated the effectiveness of a biosurfactant derived from Lactiplantibacillus plantarum on QS-regulated virulence factors and biofilm formation in Pseudomonas aeruginosa and Chromobacterium violaceum. The structural analogues of the crude biosurfactant were identified using gas chromatography–mass spectrometry (GC–MS). Moreover, the inhibitory prospects of identified structural analogues were assessed with QS-associated CviR, LasA, and LasI ligands via in silico molecular docking analysis. An L. plantarum-derived biosurfactant showed a promising dose-dependent interference with the production of both violacein and acyl homoserine lactone (AHL) in C. violaceum. In P. aeruginosa, at a sub-MIC concentration (2.5 mg/mL), QS inhibitory activity was also demonstrated by reduction in pyocyanin (66.63%), total protease (60.95%), LasA (56.62%), and LasB elastase (51.33%) activity. The swarming motility and exopolysaccharide production were also significantly reduced in both C. violaceum (61.13%) and P. aeruginosa (53.11%). When compared with control, biofilm formation was also considerably reduced in C. violaceum (68.12%) and P. aeruginosa (59.80%). A GC–MS analysis confirmed that the crude biosurfactant derived from L. plantarum was a glycolipid type. Among all, n-hexadecanoic acid, oleic acid, and 1H-indene,1-hexadecyl-2,3-dihydro had a high affinity for CviR, LasI, and LasA, respectively. Thus, our findings suggest that the crude biosurfactant of L. plantarum can be used as a new anti-QS/antibiofilm agent against biofilm-associated pathogenesis, which warrants further investigation to uncover its therapeutic efficacy.

Published in Microorganisms

ISSN: 2076-2607 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.mdpi.com/journal/microorganisms

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