Nature Communications (Dec 2016)

Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions

  • Hendrik Deschout,
  • Tomas Lukes,
  • Azat Sharipov,
  • Daniel Szlag,
  • Lely Feletti,
  • Wim Vandenberg,
  • Peter Dedecker,
  • Johan Hofkens,
  • Marcel Leutenegger,
  • Theo Lasser,
  • Aleksandra Radenovic

DOI
https://doi.org/10.1038/ncomms13693
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 11

Abstract

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Live cell super-resolution imaging requires a high temporal resolution, which remains a challenge. Here the authors combine photo-activated localization microscopy (PALM) with super-resolution optical fluctuation imaging (SOFI) to achieve high spatiotemporal resolution and quantitative imaging of focal adhesion dynamics.