Genome-wide Identification, Phylogenetic and Expression Analysis of ABC1K Gene Family in Tomato (Solanum lycopersicum L.)

Abstract

Activity of bc1 complex (ABC1K) is a protein kinase commonly found in eukaryotes and prokaryotes. It plays an important role in various developmental and physiological processes, especially critical for plant response to diverse biotic and abiotic stresses. In this study, a genome analysis was carried out and 18 genes of ABC1K family were identified in tomato. Phylogenetic results showed that these members could be classified into three groups ‒ ancestral clade, mitochondrial clade and photosynthetic-specific clade, with several subgroups based on subcellular location prediction by WoLF PSORT and all the SlABC1K proteins contained an ABC1K conserved kinase motifs-VAIK (VAVK, VAMK) and DFG (DEG). Conserved motifs analyzed by MEME program indicated that all ABC1K protein contains motif 2, 5, 6 and 8. Predictably, the SlABC1K proteins were localized in chloroplasts or mitochondria; in our analysis of expression patterns, SlABC1K genes could be detected in all tomato organs, and eight genes were specifically expressed in tomato leaf, which implied that the SlABC1Ks might be involved in energy metabolism in tomato. The expression of several genes was significantly changed under abiotic stress, implying their probability of performing various roles in abiotic stresses (NaCl, high temperature, cold, abscisic acid and salicylic acid).

Keywords

• Tomato
• ABC1K family
• Phylogeny
• Abiotic stress
• Gene function