Generation and characterization of iPS cell line (SYSUSHi002-A) from PBMCs of an AML patient with TP53 mutation

Lihua Yuan; Yaqun Wang; Rongli Xie; Dongjun Lin; Xiaobo Wang

Stem Cell Research (Dec 2023)

Generation and characterization of iPS cell line (SYSUSHi002-A) from PBMCs of an AML patient with TP53 mutation

Lihua Yuan,
Yaqun Wang,
Rongli Xie,
Dongjun Lin,
Xiaobo Wang

Affiliations

Lihua Yuan: Pediatric Purgery Department, The University of Hong Kong Shenzhen Hospital, Shenzhen, Guangdong Province 518000, China
Yaqun Wang: Hematology Department, The Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, Guangdong Province, 518107, China
Rongli Xie: Hematology Department, The Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, Guangdong Province, 518107, China
Dongjun Lin: Hematology Department, The Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, Guangdong Province, 518107, China
Xiaobo Wang: Hematology Department, The Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, Guangdong Province, 518107, China; Corresponding author.

Journal volume & issue: Vol. 73
p. 103230

Abstract

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We have generated an iPSCs line (IPS-AML2-C3, SYSUSHi002-A) from AML cells of a 71-year-old male Acute Myeloid Leukaemia patient with TP53 gene mutation (TP53: c.824G > A, p.Cys275Tyr) using episomal plasmids encoding the factors OCT4, SOX2, KLF4, L-MYC and human miR-302. The IPS-AML2-C3 (SYSUSHi002-A) iPSC line displayed typical embryonic stem cell-like morphology, carried the TP53 gene mutation, expressed several pluripotent stem cell makers, retained normal karyotype (46, XY), and was capable of forming three germ layer cells in vitro.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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