Study of the Effect of Gold Nanoparticles on HPG Axis (Hypothalamic-Pituitary-Gonad) in Syrian Male NMRI

Abstract

Introduction Nanoparticles manufactured using nanotechnology, have always been a concern because of their toxic and harmful effects, despite having numerous benefits. In this study, the effect of gold nanoparticles on LH and FSH hormones as well as testosterone was investigated in males NMRI. Materials and Methods 30 male NMRI were classified randomly into 3 groups of 10 including control and experimental groups where the mouse received 50ppm and 100ppm of nanoparticles. Then, gold nanoparticles were injected for 7 days using intraperitoneal injection and after the seven-day period, animals were anesthetized using ketamine and xylazine and blood samples are taken from heart. Then, they were dissected and testicle tissue was extracted to be prepared to perform histological studies. Results In this study, significant differences was observed in concentration of LH and FSH hormones (increased concentration) in taken doses and significant changes were observed in testosterone hormone (decrease) in doses of 100 and 50 ppm that could be because of the inhibiting effect of gold nanoparticles on the performance of testosterone producing cells. Statistical calculations were performed using the statistical software version SPSS v.21. Conclusion Results of this study showed that gold nanoparticles change the amount of LH and FSH hormones as well as testosterone in male Syrian mouse and this will result in interference with metabolism system.

Keywords

• Nanoparticles
• LH and FSH Hormones
• Testosterone
• Testicle
• Syrian Male