Generation of a human-derived induced pluripotent stem cell line (SIAISi012-A) from an 82-year-old healthy Chinese Han male

Jinghui Guo; Fang Fang; Qiuting Dai; Wenxin Zhang; Weihao Di; Jiaan Hu; Jian Zhao; Jiumei Cao; Ying Wang

Stem Cell Research (Apr 2021)

Generation of a human-derived induced pluripotent stem cell line (SIAISi012-A) from an 82-year-old healthy Chinese Han male

Jinghui Guo,
Fang Fang,
Qiuting Dai,
Wenxin Zhang,
Weihao Di,
Jiaan Hu,
Jian Zhao,
Jiumei Cao,
Ying Wang

Affiliations

Jinghui Guo: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China
Fang Fang: Department of Geriatrics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Qiuting Dai: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China
Wenxin Zhang: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China
Weihao Di: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China
Jiaan Hu: Department of Geriatrics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Jian Zhao: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China
Jiumei Cao: Department of Geriatrics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Corresponding author.
Ying Wang: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; Corresponding author.

Journal volume & issue: Vol. 52
p. 102186

Abstract

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As the global median population age increases, neurological diseases associated with aging pose significant challenges to human health. Appropriate modeling systems can be useful tools to better understand the mechanism of age-related neuronal degeneration diseases. Here, we successfully generated an iPSC-derived modeling system of an 82-year-old healthy man, this newly established line showed that all pluripotent markers were expressed, and the differentiation potential was confirmed by trilineage differentiation. STR profiling proved the cell line identity, and G-binding showed the normal karyotype.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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