Antioxidative enzymes during germination of two lines of Serbian spruce [Picea omorika (Panč.) Purkyně]

Abstract

Two lines of Picea omorika (Panč.) Purkyně were compared with respect to germination percentage as well as specific activity and isoenzyme pattern of catalase, superoxide dismutase, and peroxidase (POD) during germination. Line A had a higher germination percentage and higher enzyme activities in dry seeds and seedlings compared to line C. Peroxidase activity was not detected in dry seeds, but measured up to 10 U/g and 28 U/g on the 7th day of germination in lines C and A, respectively. The most abundant POD basic isoform in seedlings of both lines (pI 8.2) was not found previously in needles of adult Serbian spruce trees of the same lines. .

Keywords

• Picea omorika (Panč.) Purkyně
• seed germination
• catalase
• peroxidase
• superoxide dismutase