A Label-Free Aptasensor for Ochratoxin a Detection Based on the Structure Switch of Aptamer

Feng Liu; Ailing Ding; Jiushang Zheng; Jiucun Chen; Bin Wang

doi:10.3390/s18061769

Sensors (Jun 2018)

A Label-Free Aptasensor for Ochratoxin a Detection Based on the Structure Switch of Aptamer

Feng Liu,
Ailing Ding,
Jiushang Zheng,
Jiucun Chen,
Bin Wang

Affiliations

Feng Liu: Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China
Ailing Ding: Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China
Jiushang Zheng: Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China
Jiucun Chen: Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China
Bin Wang: Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China

DOI: https://doi.org/10.3390/s18061769
Journal volume & issue: Vol. 18, no. 6
p. 1769

Abstract

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A label-free sensing platform is developed based on switching the structure of aptamer for highly sensitive and selective fluorescence detection of ochratoxin A (OTA). OTA induces the structure of aptamer, transforms into G-quadruplex and produces strong fluorescence in the presence of zinc(II)-protoporphyrin IX probe due to the specific bind to G-quadruplex. The simple method exhibits high sensitivity towards OTA with a detection limit of 0.03 nM and excellent selectivity over other mycotoxins. In addition, the successful detection of OTA in real samples represents a promising application in food safety.

Published in Sensors

ISSN: 1424-8220 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology
Website: http://www.mdpi.com/journal/sensors

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