Technology for Distribution and Control of <i>Agrobacterium tumefaciens</i> in Cherry Tree Soil

Abstract

Sakura crown gall, caused by the invasion of Agrobacterium tumefaciens through plant wounds, poses a significant threat to cherry trees. In this study, the distribution of A. tumefaciens was preliminarily determined by stratified sampling and qPCR detection. Vertically, the pathogen is mainly distributed in the soil layer below 20 cm, and the amount of bacteria increases at greater depths. Horizontally, they are found within a 150 cm radius from the trunk. Zhongshengmycin and Oligosaccharide–Ethylicin were applied 100 cm from the trunk at a depth of below 20 cm. In the 20~40 cm soil layer, a 600-fold diluted solution of 3% Zhongshengmycin had a relative control efficacy of 94% to 100% against Agrobacterium tumefaciens, while a 1000-fold diluted solution of 25% Oligosaccharide–Ethylicin showed a control effect ranging from 54% to 100%. Before transplantation, the soil was disinfected with dazomet and abamectin. Application rates were 35 g/m2 for dazomet (98% granules) and 1 mL/m2 for abamectin (1.8% emulsifiable concentrate). The disinfection effectiveness was 77~100% in the 0~60 cm soil layer.

Keywords

• crown gall
• cherry tree
• <i>Agrobacterium tumefaciens</i>
• qPCR detection
• soil fumigation
• control efficiency